Rapid Gas-phase Isotopic Labeling for Enhanced Detection of Protein Conformations

A mass spectrometer (MS) that is adapted to allow rapid gas- phase hydrogen/deuterium exchange (HDX) labeling of ions in one or more traveling wave ion guides (TWIGs) with or without ion mobility separation. The addition of isotopic labeling by gas- phase HDX offers a sensitive alternative dimension for conformational detection, which enables high resolution detection of gaseous conformations based on shape and surface reactivity. Gas-phase, isotopic HDX labeling, or "curtain" labeling, can be performed by infusing a reactive, isotopic labeling gas, e.g., ND3, into one or more of the traveling-ion wave guides (TWIG) in the MS. Analyte ions retained in the potential wells of a traveling wave generated by one or more of the TWIGs can be isotopic labeled at adjustable gas pressures. Labeling times can also be controlled by adjusting the speed of the traveling wave and can be performed within milliseconds of ionizations, probing protein conformations present in solution.

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Patents:
WO 2,010,120,895

Inventor(s): RAND KASPER D [US]; ENGEN JOHN R [US]; BATEMAN ROBERT H [GB]

Type of Offer: Licensing



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