Determination of Dissociation Constants Using Piezoelectric Microcantilevers

A method for determining the dissociation constant (Kd) by plotting resonance frequency shift as a function of time for various target analyte concentrations. From this graph, the fraction of saturation, i.e. equilibrium fraction of bound binding sites out of all available binding sites on the sensor surface may be estimated by taking the ratio of the equilibrium resonance frequency shift at a selected concentration to the equilibrium frequency shift of the sensor. The dissociation constant is the inverse slope of the line produced by graphing the fraction of saturation as a function of concentration. This method is particularly useful for the study of protein-protein and protein-mRNA interactions.

Patents:
US 20,110,086,435

Inventor(s): SHIH WAN Y [US]; CAPOBIANCO JOSEPH [US]; SHIH WEI-HENG [US]

Type of Offer: Licensing



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