Methods for Determination of Protein Phosphatase Activity, and Uses in Predicting Therapeutic Outcomes

One aspect of the present disclosure encompasses methods for determining a protein kinase or phosphatase activity in a biological sample, comprising: contacting in a reaction mix a first test sample and a fluorescently-labeled peptide substrate capable of being modified by a protein phosphatase or a protein kinase, contacting the reaction mix with a TiO2 matrix, thereby partitioning fluorescently-labeled phosphorylated peptide from fluorescently-labeled dephosphorylated peptide; and determining the fluorescence of the fluorescently-labeled dephosphorylated peptide, thereby determining a protein kinase or phosphatase activity.

Patents:
WO 2,009,018,424

Inventor(s): GOOCH JENNIFER L [US]; ROBERTS BRIAN R [US]; POHL JAN [US]

Type of Offer: Licensing



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