Fibrous Assemblies for Antibody Presentation, and Multiplexed Antigenic Analysis Using Same

Biofunctionalized fibers including a fiber platform and a histidine-tagged protein and, optionally, an antibody. Chitosan is a fiber useful as the fiber platform. The fiber platform may be treated with nickel or may be directly linked to the histidine-tagged protein e.g., histidine-tagged streptococcal IgG-binding protein, protein G, protein G3T, GFP or RFP. The resulting biofunctionalized fibers can be assembled into protein fiber assemblies by a variety of biofabrication methods. The fiber assemblies, e.g., in the form of woven fabrics, are useful for (i) antigen capture; (ii) immunoanalysis, and/or (iii) multiplexed analysis.; In one fabrication, each fiber of a fiber assembly presents a specific antibody, and mixing and matching of fibers, for example by weaving of fabrics from various antibody-presenting fibers, allows for multiple antigens to be captured simultaneously for multiplexed analysis.

Attached files:


Patents:
WO 2,009,137,211

Inventor(s): SHI XIAO-WEN [US]; WU HSUAN-CHEN [US]; PAYNE GREGORY F [US]; BENTLEY WILLIAM E [US]

Type of Offer: Licensing



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