Droplet-Based Pyrosequencing

The present invention relates to droplet-based pyrosequencing including a method of identifying a base at a target position in a sample nucleic acid. The method includes: (a) providing a droplet microactuator including a first droplet including a sample nucleic acid immobilized on a bead; and (b) on the droplet microactuator: (i) contacting the first droplet with one or more reagent droplets to yield a second droplet, wherein the one or more reagent droplets include reagents for extending a double stranded portion of the sample nucleic acid by incorporating a nucleotide at the target position; (ii) splitting the second droplet to yield a third droplet including the bead and a fourth droplet lacking the bead; and (iii) assaying the third droplet to determine whether the nucleotide was incorporated at the target position.

Attached files:


Patents:
US 20,100,291,578

Inventor(s): POLLACK MICHAEL G [US]; PAMULA VAMSEE K [US]; ALLEN DWAYNE J [US]; FAIR RICHARD B [US]; GRIFFIN PETER [US]

Type of Offer: Licensing



« More Chemistry Patents

---