Problem Solver

John Pisciotta, PhD star Proven Solver

John Pisciotta, PhD
I specialize in clean energy and biotechnology and enjoy working with people who are interested in cleantech. Trained in wind and solar PV energy systems and hold a PhD with a focus on lipid/oil synthesis in microorganisms. I have patent writing experience.

Areas John Pisciotta, PhD is Knowledgeable in:

Clean Energy, Biotechnology, Algae, Photosynthesis, Lipid and oil synthesis in cells, Solar electricity, Fuel Cells, environmental remediation.

Medical diagnostics and antibiotic drug development.

Techniques John Pisciotta, PhD Uses:

Patent searches, bacterial, algal and protozoan cell culture. Metabolomic profiling of microorganisms. Mass spectrometry, chromatography, FAME lipid analysis, DGGE microbial community analysis. Microbial fuel cells. Fluorescence microscopy, antimicrobial susceptibility testing and drug discovery, electron microscopy, SEM/TEM sample processing, UV-Vis spectroscopy, FTIR, subcellular fractionation techniques, RT-PCR, gene cloning, recombinant protein expression and purification. Biochemical assay development. Western blot, ELISA and immunochromatographic diagnostics. Experience with electrochemical methods cyclic voltammetry and impedance spectroscopy. Prototyping.

John Pisciotta, PhD's Problem Solving Skills:

  1. IP/tech transfer, Cleantech and Biotech. Specialize in microbial physiology, lipid synthesis and algae.

John Pisciotta, PhD's Problem Solving Experience:

  1. Helped develop Photosynthetic Microbial Fuel Cells to convert sunlight to electricity using algae. (As published in: Bioelectrochemistry, PloS One and Biotechnology + Bioengineering)

    Helped win "Extraction of Specific Proteins from Oilseeds" contest.

    Ran large Aquaculture project for Maryland Dept. of Natural Resources (DNR).

    Invented Clonal Replication System (US patent #6,753,180) to improve replication of microbial colonies.

    Invented Heat exchanging apparatus for handling and transporting vessels (US patent 6,701,718).

    Invented Metabolite transfer disk technology presented at 11th annual MIT Venture Capital Conference in 2008.

    Invented Rip-current broken wave energy converter (Semi-finalist in US Dept. of Energy’s 2008 I2P clean energy contest (Johns Hopkins University team).

    Invented Window-insulating solar generators to increase solar footprint of buildings while reducing heat loss.