Problem Solver
Muhammad Asif
Areas Muhammad Asif is Knowledgeable in:
Forensic DNA Typing, Parentage Analysis
Techniques Muhammad Asif Uses:
Development of new miniSTR markers
Seven new miniSTR markers have been developed and placed in 7plex. These miniSTR loci are unlinked to CODIS or previously published miniSTR loci.
1. Uniprime2 (Program for Universal Primer designing)
2. Primer3 (Program for designing of new miniSTR primers)
3. BLAT / BLAST (Program for identification of chromosome location)
4. AutoDimer (Program for testing primers for multiplex-ability)
5. OligoTM 1.0 (Program for calculation oligo melting temperature and GC content)
6. OligoCalc: (Oligonucleotides Properties Calculator)
7. SNPmasker 1.0 (Program for automatic masking of SNPs and repeats across eukaryotic genomes)
Seven new miniSTR markers have been developed and placed in 7plex. These miniSTR loci are unlinked to CODIS or previously published miniSTR loci.
1. Uniprime2 (Program for Universal Primer designing)
2. Primer3 (Program for designing of new miniSTR primers)
3. BLAT / BLAST (Program for identification of chromosome location)
4. AutoDimer (Program for testing primers for multiplex-ability)
5. OligoTM 1.0 (Program for calculation oligo melting temperature and GC content)
6. OligoCalc: (Oligonucleotides Properties Calculator)
7. SNPmasker 1.0 (Program for automatic masking of SNPs and repeats across eukaryotic genomes)
Muhammad Asif's Problem Solving Skills:
- DNA reports writing and court testimony etc
- DNA reports technical review
- DNA quantification with Human DNA Quantification Kit, Human DNA Quantifiler Duo Kit,
- DNA mixture analysis
- DNA extraction from bones, teeth etc
- forensic Serology
- DNA Extraction from forensic samples
- Forensic DNA Typing
- STR Analysis with Identifiler, Power Plus 16, Yfiler, miniFiler
- Paternity, Maternity, Sibship Analysis
Muhammad Asif's Problem Solving Experience:
- 1. Have the experience examining physical evidence using analytical instruments and forensic science techniques.
2. Have fair knowledge of forensic laboratory policies, procedures and guidelines.
3. Have the ability to organize, assemble and write detailed technical reports based on the results of scientific examinations.
4. Extraction and purification of DNA from whole blood, bones, teeth, hair, nail, saliva, seminal fluid, vaginal section, skin cells and other biological samples by organic, Differential and other current validated methods
5. DNA quantification with spectrophotometer, Real Time PCR using Human DNA Quantifiler and Quantifiler Duo kit
6. PCR amplification of DNA and troubleshooting
7. Genotyping analysis on ABI Prism 310, 3130 Genetic Analyzer, using Identifiler, Yfiler, miniFiler, and PowerPlus -16