Problem Solver

Nicholas Young

Areas Nicholas Young is Knowledgeable in:

I have extensive experience in molecular biological analysis and interpretation in the investigation of cancer, microbial infections, and immunological disease.

Nicholas Young's Problem Solving Skills:

  1. • In-depth in vitro and in vivo scientific analysis in the research and investigation of disease in cancer and immunological
  2. • Experience in human subject research and analysis Proficient in Microsoft Office software especially Excel, Word, and Power
  3. • Acting clinical research coordinator of a human subject protocol of both Lupus and healthy patients at The Ohio State Unive
  4. • Extensive work with mouse models of disease and the work involved with breeding and maintaining mouse lineages
  5. • Leadership of team-oriented projects, formulation and institution of solutions to increase lab-wide productivity, hard-work
  6. • Advanced microbiological techniques from a variety of patient sources including Vitek processing and analysis, occult blood
  7. • Microscopy experience including: fluorescence microscopy, digital imaging and image analysis; as well as in the analysis of
  8. • Radiation experimental work with both 32P and 3H in cancer cell lines
  9. • Extensive work with ELISA and Flow Cytometry processing and analysis. Data acquisition and analysis with Cell Quest and Fl
  10. • Tissue/cell culture techniques including: stable cell line creation with siRNA primer design, analysis, and plasmid prepara
  11. • Advanced molecular techniques including Real-Time PCR (Taq Man), DNA sequencing, plasmid construction and preparation, West
  12. • Experimental planning and assay development, project design, manuscript and grant writing, presentation of work in both a f
  13. • Over ten years of intensive biological research investigation in an academic setting and success in transition to a high vo
  14. • Follows S.O.P. in industrial setting exceptionally, keeps excellent and organized documentation, maintains clean laboratory
  15. • Proficient in digital image analysis software as used for densitometry and scaling/measuring captured microscope images esp

Nicholas Young's Problem Solving Experience:

  1. Publications:

    1. Young, N., Friedman A.K., Sharma R., James H., Kaffenberger B., Jarjour W.N.. The Suppressive Influence Regulatory T Cells and the Role of Endogenous Muscle Tissue Antigens in Directing the Development of Autoimmune Myopathy in Mice. J Autoimmun. In review
    2. Young N., James H., Shupnik M., Friedman A.K., Sharma R., Kaffenberger B., Jarjour W. N. Influence of Estrogen to the Gender-Biased Pathology in Patients with Systemic Lupus Erythematosus. Nat Med. In review
    3. Young, N. and J.R. Van Brocklyn. Essential Role Of Sphingosine Kinase And Sphingosine 1-Phosphate Receptor Subtypes In Urokinase Plasminogen Activator And CCN1 Mediated Glioblastoma Multiforme Invasiveness. Mol Cancer Res 2009;7(1). January 2009
  2. Continued
    4. Young, N. Regulation of Invasiveness of Glioblastoma Multiforme Cell Lines and Tumor Stem Cells by Sphingosine Kinase and Sphingosine 1-Phosphate Reveals Potential Molecular Therapeutic Targets. 21st annual Edward F. Hayes Graduate Research Forum Proceedings. The Ohio State University Libraries Knowledge Bank. https:\\kb.osu.edu/dspace/handle/1811/29390. August 2007.
    5. Young, N. and Van Brocklyn, J.R. (2007) Roles of sphingosine-1-phosphate (S1P) receptors in malignant behavior of glioma cells. Differential effects of S1P(2) on cell migration and invasiveness. Exp Cell Res,. 313(8): 1615-27.
    6. Young, N. and Van Brocklyn, J.R. (2006) Signal Transduction of Sphingosine-1-Phosphate G Protein–Coupled Receptors. TheScientificWorldJournal 6, 946-966. DOI 10.1100/tsw.2006.182
    7. Van Brocklyn JR, Young N, Roof R. (2003) Sphingosine-1-phosphate stimulates motility and invasiveness of human glioblastoma multiforme cells. Cancer Lett. Sep 10;199(1):53-60.