1. Plant Biotechnology
2. Plant Tissue Culture
3. GM Crop Production
4. Molecular Analysis of Transgenic Plants
5. Trouble Shooting of steps involved
Techniques Parashuram Joger Uses:
My Experience and Literature Support
Parashuram Joger's Problem Solving Skills:
Plant Tissue Culture and Transformation, GM Crops
Parashuram Joger's Problem Solving Experience:
I. Established regeneration & transformation protocol to produce Bt Cotton through
Agrobacterium-mediated transformation method.
a. Co-culture with embryo
b. Co- culture with tissue explants. c. In Planta transformation.
II. Established a regeneration and Transformation protocol for Withania somnifera. Dunal resulting in 3-fold increase in Secondary Metabolite Production as detected through HPLC. (Ph.D Work).
a. Established In vitro and In Planta transformation protocols for Brinjal and Tomato and confirmed tangents in T1 generations.
b. Established non-toxic Xylose isomerase selection transgenics.