Problem Solver

Robert Ollar

Areas Robert Ollar is Knowledgeable in:

Global TB and Mycobacterial Infections, Molecular Biology of TB and Mycobacterial Diseases, Application of Molecular Biology for Early Detection of Fungal Pathogenes
Applicaton of Immunological and Molecular Technologies for detection gastrointestinal pathogens (ie E. coli, V.cholorerae. Listeria monocytogenes)

Applicaton of Molecular Based Systems for early detection in cancer (ie Colon Ca, and Pancreatic Ca)

Techniques Robert Ollar Uses:

Classical Microbiology Techniques involving Culture and Microscopy
Molecular Biological Methodologies (PCR, Electrophoresis, Electroporation. Cloning, ELISA, Colorimetric and Fluorometry Labelling and Detection, Southern Blotting, Microtitre Plate Assays, Reatime PCR, Micro Arrays)

Immunology, Monclonal Antibodies, ElISA, Column Chromatographic Separations, Gel Diffusion, Western Blotting, Immunofluorescence

Immunohistochemistry, paraffin and frozen section preparations

Robert Ollar's Problem Solving Experience:

  1. I have developed a novel and simple and low tech way to distinguish , isolate and perform antibiotic sensitivity assay for organisms that a cousins to TB (AIDS-Related Mycobacteria) and occur concomitantly in AIDS patient with TB but are different to TB and require different kinds of antibiotics. The technique is based on the fact that TB cannot utilize paraffin wax as a sole carbon source, and cousin organisms AIDS-Related Mycobacteria can utilized paraffin wax as a sole carbon source. This technology is currently being utilized in India to deal with these AIDS-Related organisms.