Problem Solver
Willson Thamalapakula
Areas Willson Thamalapakula is Knowledgeable in:
-Recombinant Protein Purification.
-Construction of Cloning and Expression Vectors.
-Selection of Vectors and E.coli Strains.
-Method Development.
-Construction of Cloning and Expression Vectors.
-Selection of Vectors and E.coli Strains.
-Method Development.
Techniques Willson Thamalapakula Uses:
-Developing or choosing appropriate methods for extraction.
-Finding conditions under which the Protein of Interest(POI) can be stored safely between steps.
-Establishing conditions under which the POI is stable and biologically active.
-Choosing or developing a sensitive, reproducible, and selective assay for the
protein of interest (POI).
-Solving the issues related to Biased errors.
-Precision and Accuracy in Methods.
-Maintaining least Error rates.
-Finding conditions under which the Protein of Interest(POI) can be stored safely between steps.
-Establishing conditions under which the POI is stable and biologically active.
-Choosing or developing a sensitive, reproducible, and selective assay for the
protein of interest (POI).
-Solving the issues related to Biased errors.
-Precision and Accuracy in Methods.
-Maintaining least Error rates.
Willson Thamalapakula's Problem Solving Skills:
- FPLC/HPLC
- SDS PAGE
- Reverse Phase Liquid Chromatography
- Affinity Chromatography
- Protein Expression Studies
- Construction of Cloning and Expression Vectors
- Process Development for the Purification of recombinant Proteins
- Lyophilization
- Development of Radioimmunoassay
Willson Thamalapakula's Problem Solving Experience:
- I developed better Expression system for FI gene in pET28series expression vector in modified E.coli.
- I developed a Stable Enfuvirtide molecule by developing a efficient Formulation.
- I developed a Purification Protocol for the recombinant Enfuvirtide produced from Prokaryotic System.
- I developed a Solid Phase Radio immunoassay for Testosterone in Human Serum.
- I developed Monoclonal Antibodies against Human Testosterone.