Problem Solver

Wong Siu Tak

Areas Wong Siu Tak is Knowledgeable in:

Biochemistry, Biotechnology, Biological signaling pathways

Techniques Wong Siu Tak Uses:

ELISA, Western blot, SDS-PAGE, Mass Spectrometry, enzyme activity assays, RT-PCR, RNA purification, protein purification, sperm-hemizona binding assay

Wong Siu Tak's Problem Solving Skills:

  1. RNA purification
  2. protein purification
  3. Sperm purification
  4. Sperm-oocyte binding
  5. Western blot
  6. SDS-PAGE protein analysis
  7. ELISA
  8. siRNA transfection

Wong Siu Tak's Problem Solving Experience:

  1. I have discovered glycodelin-A as a protein that exerts a priming effect on human sperm acrosome reaction.
  2. I identified a anti-bovine-ZP3 antibody that can also recognize the human-ZP3 protein. This provides the possibility to purify human ZP3 proteins from human oocyte using anti-bovine-ZP3 instead of anti-human-ZP3 antibody. This is important as the cost of producing anti-bovine-ZP3 is much less than that of producing anti-human-ZP3.