ELISA, Western blot, SDS-PAGE, Mass Spectrometry, enzyme activity assays, RT-PCR, RNA purification, protein purification, sperm-hemizona binding assay
Wong Siu Tak's Problem Solving Skills:
SDS-PAGE protein analysis
Wong Siu Tak's Problem Solving Experience:
I have discovered glycodelin-A as a protein that exerts a priming effect on human sperm acrosome reaction.
I identified a anti-bovine-ZP3 antibody that can also recognize the human-ZP3 protein. This provides the possibility to purify human ZP3 proteins from human oocyte using anti-bovine-ZP3 instead of anti-human-ZP3 antibody. This is important as the cost of producing anti-bovine-ZP3 is much less than that of producing anti-human-ZP3.