Development of molecular assay for pathogen detection.
Identification of bacteria using 16S rRNA
Genotyping (ERIC, VNTR, MLST)
Yogesh Chander's Problem Solving Experience:
I proved that antibiotics (tetracycline and tylosin) adsorbed on soil particles still retian their antimicrobial activity.
I developed a multiplex PCR for identification of seven antibiotic resistant genes (four tetracycline: tetA, tetB, tetL, tetM and three beta-lactamase: blaTEM-1, blaCMY-2, blaROB-1).
I identified tetracycline resistance gene, tetB, in Streptococcus suis. This is the first report on identification of this gene in Gram-positive bacteria.
I developed a set of degenerate primers for full length amplification of four genes of avian influenza virus in a single reaction. This is unique as a single set of primers can amplify four different genes in a single reaction.
I developed and standardized Milk ELISA test for the detection of Johne’s disease in dairy cattle.
I developed an RT-PCR protocol for full length amplification of neuraminidase (NA) gene of avian and swine influenza viruses.
I standardized use of a 16S rRNA technique for the identification of bacteria at the Minnesota Veterinary Diagnostic Laboratory (MVDL), University of Minnesota
I studied effect sub-therapeutic use antibiotics on animal farms on the emergence and spread of antibiotic resistant bacteria (ARB) in the environment.