Membrane Protein Expression System and Its Application in Drug Screening

PROBLEM TO BE SOLVED: To provide a convenient in vitro membrane protein expression system using an inhibitor of drug efflux pump proteins and heterologous cells for testing other drug resistance-relating cell membrane proteins. ; SOLUTION: Provided is a protein expression system comprising an in vitro cell-based expression system for over-expression of a heterologous protein having efflux pump action relating to the drug resistance of a host cell membrane for drug screening applications, that is to say, a protein expression system comprising: (i) a host yeast cell; and (ii) a vector comprising the coding sequence of a target heterologous membrane protein, wherein the sequence is under the control of a promoter which, upon transformation of the host cell and chromosomal integration, causes over-expression of the functional target protein in the membrane of the host cell. ; COPYRIGHT: (C)2010,JPO&INPIT

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Patents:
JP 2,010,011,851

Inventor(s): MONK BRIAN CHARLES; CANNON RICHARD DAVID; NAKAMURA KENJIRO; NIIMI SHOICHI; NIIMI KYOKO; HOLMES ANN RACHEL; LAMPING ERWIN; HARDIN

Type of Offer: Sale



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