Method for Releasing and Labelling 0-glycans
The application describes a one-pot process for releasing and labelling O-glycans from glycoproteins, thereby facilitating analysis via LC-ESI-MS-MS. The process comprises contacting the glycoprotein with an amine, eg. methylamine, dimethylamine, or ammonia, NH3 in the presence of a labelling agent, especially l-phenyl-3-methyl-5-pyrazolone (PMP,- CAS Reg. No. 89-25-8), l-naphthyl-3-methyl-5-pyrazolone (NMP) or 1- (4-methoxy)phenyl-3-methyl-5-pyrazolone (PMPMP, CAS Reg. No. 60798-06-3).
WUHRER MANFRED [NL]; ZAUNER GERHILD [NL]
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