Scalable Process for Producing Phycocyanine

The invention relates to a three-stage process for producing and purifying phycocyanine originating from microalgae of the genus Anabaena, said process being characterised by scalability and high yield. The first stage consists of a cell disruption by means of an osmotic shock which releases the cytoplasmatic material, using a phosphate buffer. The second stage uses a chromatographic expanded-bed adsorption column consisting of an ion-exchange material as the adsorbent phase. The third stage is an additonal chromatographic process in a column of ion-exchange material using an anion exchanger as a static phase, operating in a packed format. In optimum conditions, the yield following the second stage corresponds to approximately 87 % recovery of phycocyanine, while the total yield of the process following the third stage corresponds to 64 % of pure phycocyanine.

Patents:
WO 2,009,050,319

Inventor(s): BERMEJO ROMAN RUPERTO [ES]; ACIEN FERNANDEZ FRANCISCO GABRIEL [ES]

Type of Offer: Sale



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