Scalable Process for Producing Phycocyanine
The invention relates to a three-stage process for producing and purifying phycocyanine originating from microalgae of the genus Anabaena, said process being characterised by scalability and high yield. The first stage consists of a cell disruption by means of an osmotic shock which releases the cytoplasmatic material, using a phosphate buffer. The second stage uses a chromatographic expanded-bed adsorption column consisting of an ion-exchange material as the adsorbent phase. The third stage is an additonal chromatographic process in a column of ion-exchange material using an anion exchanger as a static phase, operating in a packed format. In optimum conditions, the yield following the second stage corresponds to approximately 87 % recovery of phycocyanine, while the total yield of the process following the third stage corresponds to 64 % of pure phycocyanine.
BERMEJO ROMAN RUPERTO [ES]; ACIEN FERNANDEZ FRANCISCO GABRIEL [ES]
Type of Offer:
« More Chemistry Patents