Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.
WANGH LAWRENCE J [US]; RICE JOHN [US]; SANCHEZ J AQUILES [US]; PIERCE KENNETH [US]; SALK JESSE [US]; REIS ARTHUR [US]; HARTSHORN
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