Method for Imaging on Thin Solid-state Interface Between Two Fluids

Described herein is a fluid cell for an optical microscopy tool having a solid state membrane having a first side and a second, opposing side; a first fluid chamber comprising a first fluid having a first refractive index located on the first side of the membrane; and, a second fluid chamber comprising a second fluid having a second refractive index located on the second side of the membrane, the second refractive index being different than the first refractive index. Also described herein is a method for imaging a single biomolecule, the method including generating a field of evanescent illumination at a solid state membrane between a first fluid and a second fluid having different refractive indexes; and detecting light emitted by optical detectors linked to the single biomolecules at the solid state membrane.

Attached files:
WO 2010111602.jpg

WO 2,010,111,602


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