Nucleic Acid Expression Construct and Its Use As a Cell Proliferation Marker
In a first aspect, the present invention provides a nucleic acid expression construct encoding a fusion protein comprising a reporter protein and a protein with a wild-type destruction signal, wherein the sequence encoding the fusion protein is operably linked to a non-endogenous promoter, and wherein the fusion protein localizes during cell cycle progression to subcellular structures selected from the group consisting of the cell cortex, the contractile ring, and the midbody. In a further aspect, the present invention provides a method of visualizing cell cycle progression, In another aspect, the present Invention provides the use of the nucleic acid expression construct of the present invention as a cell proliferation marker in vivo and in vitro. In another aspect, the present invention provides the use of the nucleic acid expression construct of the present invention to distinguish between cell division and acytokinetic mitosis and/or endoreduplication in vivo or in vitro. In yet another aspect, the present invention provides a transgenic animal comprising the nucleic acid expression of the present invention as well as its use for analyzing cell proliferation in vivo.
FLEISCHMANN BERND K [DE]; HESSE MICHAEL [DE]; RAULF ALEXANDRA [DE]
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