Method for Obtaining Human Microglial Precursor Cells from Pluripotent Stem Cells
The present invention relates to a method for obtaining human microglial precursor cells, comprising: (a) providing a ceil population comprising neural precursor cells, wherein the cell population is obtainable from embryoid bodies differentiated from human pluripotent stem cells; (b) differentiating the cell population comprising neural precursor cells into microglial precursor cells by culturing in medium comprising a growth factor selected from the group consisting of insulin and insulin-like growth factors; (c) expanding and enriching microglial precursor cells in medium comprising a growth factor selected from the group consisting of insulin and insulin-iike growth factors and 10 to 150 ng/ml GM-CSF; and (d) isolating microglial precursor cells comprising CD45-positive cells.
NEUMANN HARALD [DE]; ROY KRISTIN [DE]; BRUESTLE OLIVER [DE]; PEITZ MICHAEL [DE]
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