Media Conditioning for Improving Gene Delivery Efficiency to Differentiating Embryonic Stem Cells
The present invention provides systems and methods for improving the efficiency of a transient gene delivery system to differentiating embryonic stem (ES) cells by serum starving the targeted cells for one to three days prior to transfection. Such a serum starvation surprisingly resulted in increased expression of a constitutively-controlled plasmid from 50.4% to 83.2% of the population and increased expression of a promoter/enhancer controlled plasmid from ~1.4% to ~3.7% of the population.
YARMUSH MARTIN L [US]; WALLENSTEIN ERIC J [US]; SCHLOSS RENE S [US]
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