Fluorescent in Situ PCR

Research Tools-Diagnostics

In situ PCR (polymerase chain reaction) has become a powerful molecular tool in research as well as clinical practice. This technique has resulted in an increased understanding of infectious and neoplastic diseases and improvements in diagnosis of disease. In situ RT-PCR (Reverse Transcription- PCR) gives more detailed information by allowing for highly sensitive detection of low abundance gene expression in a given cell while providing anatomical information. The usefulness of these techniques has been hampered by low detection sensitivity, poor reproducibility, and high backgrounds. Moreover, many of the methods used to visualize the results of PCR amplification within cells and tissues employ radioactive tracers, making performance of the techniques cumbersome and costly.

IU researchers have developed a method for specific fluorescent detection of gene expression using in situ RT-PCR. This method enables the researcher or clinician to detect low levels of gene expression within tissues with very low background interference while addressing many of the other existing drawbacks to using in situ RT-PCR.

* Low background
* High specificity
* Fast assay with shorter turn-around time
* No radioactive chemicals needed

US 7,186,507

Inventor(s): Dr. Robert Bacallao

Type of Offer: Licensing

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