Novel Biosensors for Live Cell Imaging of Intracellular Proteins
This invention is related to the field of imaging intracellular protein processing. Fluorescent probes capable of changing color over time are used to identify and track the synthesis, trafficking, and secretion of intracellular proteins. One such probe useful in this invention is a monomeric multifluorescent protein, specifically a mcTimer probe as a biosensor for secretory proteins. After expression, mcTimer sequentially changes from green to red over a twenty- four hour period. In particular, a monomeric multifluorescent protein is inserted into the C-linker portion of the insulin prepropeptide as a marker for normal insulin responses to glucose in cultured donor beta cells.
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