Method and Means for Activating Memory B Lymphocytes
The invention relates to a method and means for activating memory B lymphocytes, in particular for use in immunological research and in medicine. For the inventive method, the cells are treated with an activating reagent containing pokeweed mitogen, CpG oligonucleotides, IL-2 and IL-10. It is surprising that IL-2, IL-10 and CpG oligonucleotides enhance the activating effect of pokeweed mitogen. The inventors have found that the addition of IL-6, LPS, CD40 ligand or anti-CD40 antibodies, does not have a further activating effect on memory B lymphocytes. This is surprising, because IL-6 is a growth factor for B lymphocytes. According to the invention, therefore, IL-6, lipopolysaccharide of Escherichia coli (LPS), interleukin 6 (IL-6), CD40 ligand and anti-CD40 antibodies are not contained in the activation reagent.
Type of Offer:
« More Genetics Patents