Simultaneous Quantitative Multiple Primer Detection of Clostridium Difficile
This invention comprises a multiplex-capable oligonucleotide which is capable of hybridizing to at least one of the C. difficile tcdB, tcdC, or cdtB genes, wherein, wherein said primer consists of a sequence selected from the group consisting of SEQ ID NOS: 1 through 9, or a sequence that exhibits no more than one substitution of a base to a sequence selected from the group consisting of SEQ ID NOS: 1 through 9 and method for polymerase chain reaction (PCR) determining of the presence of a toxigenic strain of C. difficile in a biological sample utilizing said probes.
TRIPATHI ANUBHAV [US]; MERMEL LEONARD [US]; SARMA AARTIK [US]; NOVIKOV ALEKSEY [US]; SEWARD LEAH [US]; FIEBER JENNIFER [US]
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