A Dna Replicon System for High-level Rapid Production of Vaccines and Monoclonal Antibody Therapeutics in Plants
Plant viral vectors have great potential in rapid production of proteins, but no simple Here a geminivirus-based system for high-yield and rapid production of oligomeric protein complexes, including virus-like particle (VLP) vaccines and monoclonal antibodies (mAbs) is described. In particular, a single vector that contains two non-competing replicons for transient expression in Nicotiana benthamiana leaves is described. The correct assembly of these subunit proteins into functional oligomeric structures (VLPs or full-size mAb) is also described. This system advances plant transient expression technology by eliminating the need for non-competing viruses, and thus, enhances the realistic commercial application of this technology for producing multiple-subunit protein complexes.
MASON HUGH S; HUANG ZHONG; CHEN QIANG; ARNTZEN CHARLES J; YUAN SHUO; HJELM BROOKE
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