Targeting and Enhancing RNA-RNA Recombination

Abstract
This invention concerns an RNA-RNA construct and a method for increasing recombination frequency when the RNA is replicated. A first RNA molecule is derived from a single stranded RNA virus such that it has a non-naturally occurring sequence at least 25 nucleotides long upstream of a set of sequences active in binding an RNA polymerase. The non-naturally occurring sequences are complementary in an antisense orientation to a sequence in a second target RNA molecule also derived from a single stranded RNA virus. The RNA molecules form a heteroduplex thus enhancing recombination. In general, this technology allows for utilization of RNA virus infections to modify the expression of host or viral genes.

Potential commercial uses and users:
Inducing viral resistance in transgenic plants. Activation of selected host genes (e.g., fluorescent proteins) triggered by viral infection. Activation or amplification of ribozymes. Gene therapy.

Advantages:
This targeting system is easy to design and use. It makes possible in planta ad hoc generation of mRNA sequences which otherwise would be difficult or impossible to obtain.

Patents:
US 5,877,401

Inventor(s): Jozef J. Bujarski, Peter D. Nagy

Type of Offer: Licensing



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