Targeting and Enhancing RNA-RNA Recombination

This invention concerns an RNA-RNA construct and a method for increasing recombination frequency when the RNA is replicated. A first RNA molecule is derived from a single stranded RNA virus such that it has a non-naturally occurring sequence at least 25 nucleotides long upstream of a set of sequences active in binding an RNA polymerase. The non-naturally occurring sequences are complementary in an antisense orientation to a sequence in a second target RNA molecule also derived from a single stranded RNA virus. The RNA molecules form a heteroduplex thus enhancing recombination. In general, this technology allows for utilization of RNA virus infections to modify the expression of host or viral genes.

Potential commercial uses and users:
Inducing viral resistance in transgenic plants. Activation of selected host genes (e.g., fluorescent proteins) triggered by viral infection. Activation or amplification of ribozymes. Gene therapy.

This targeting system is easy to design and use. It makes possible in planta ad hoc generation of mRNA sequences which otherwise would be difficult or impossible to obtain.

US 5,877,401

Inventor(s): Jozef J. Bujarski, Peter D. Nagy

Type of Offer: Licensing

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