Method for Genetic Manipulation of Extremophiles
Provided herein are methods for introducing a polynucleotide into an extremophile, such as Pyrococcus fitriosis, by conjugation with a member of the family Enterobacteriaceae. Also provided are genetically engineered extremophiles, such as Pyrococcus furiosiis, that include an exogenous polynucleotide integrated into genomic DNA.
ADAMS MICHAEL W W [US]; YANG FEI [US]; WESTPHELING JANET [US]
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