Isolation of Biological Materials
Currently, flow cytometry is the only method available for the purification of specific chromosomes.
The current flow cytometry method requires a long time to sort large quantities of cellular starting material for a single chromosome. Furthermore, species with similarly sized chromosomes, such as the mouse, make separating individual chromosomes by DNA content alone often impossible. Also, the debris and crosscontamination in sorted preparations can compromise their final utility. Technology: However, University of California researchers have combined several approaches to develop a novel chromosome isolation protocol. This method utilizes a series of straightforward chemical and physical conditions to quickly, isolate specific chromosomes with high efficiency.
This new method for chromosome sorting improves efficiency and yields, by decreasing aggregation and accessibility concerns, while increasing specificity and speed. Thus, a method to reliably generate single chromosome preparations in a short time has many applications in library construction, cloning and probe design. Application: Advantages include rapidity, the potential for high yields, and the ability to eliminate contaminating cellular material
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