A novel system for rapid fluorescence detection of non-amplified nucleic acid sequences
The Invention A new homogeneous technique which utilizes fluorescence resonance energy transfer (FRET) to directly detect and distinguish specific DNA sequences without requiring either an amplification step or a separation step has been developed. The detection system is capable of detecting very low abundant point mutations directly from unamplified genomic DNA. Furthermore, the system is able to separate the fluorescence signal from the background noise, thus achieving a dramatic improvement in the detection sensitivity. Potential Applications Non-invasive method of fetal genotyping. Medical diagnosis of genetic diseases (i.e. cystic fibrosis, Alzheimer's, sickle cell anemia and cancers). Diagnosis of pathogens. Rapid detection of chick gender in ovo.
Advantages High sensitivity Rapid detection, potentially real-time No amplification step No separation step
Stage A prototype system for synchronous detection at 488 nm is being developed. Preliminary results have demonstrated the direct detection of 6-FAM in water at 10-12 Molar. Patent filed Tech Transfer Officer Ms. Irit Ben-Chlouch Office: +972-3-6406601 Fax: +972-3-6406675 Mail: firstname.lastname@example.org
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