High-throughput hydrogels for cell culture in realistic tissue stiffness environments

Summary Markets/ Needs Addressed: The physical environment surrounding a living cell influences its ability to proliferate, metabolize, differentiate and survive. In particular, the stiffness of the underlying matrix greatly affects cell fate and behavior. For example, cultured stem cells undergo neurogenic differentiation on soft substrates (which mimic soft brain tissue), whereas on hard substrates (which mimic bone) they differentiate into bone-like cells. It is becoming increasingly clear that hydrogel substrates that mimic tissue stiffness can recapitulate key aspects of cell behavior not seen in traditional rigid tissue culture plates. Recently, a number of synthetic matrices such as PuraMatrix, AlgiMatrix and Glycosan have become commercially available. However, these products are either very costly, difficult to use, or incompatible with many cell-based high-throughput assays. An inexpensive and easy-to-use system capable of reproducing the physical environment of various tissues is in great demand for use in high-throughput cell culture, drug screening and toxicity assays.

Applications Innovation and Advantages: This invention is an economical and easy-to-use substrate system that reproduces the stiffness of the cell’s in vivo microenvironment. Matrices of various elastic properties that mimic the stiffness of different tissues in the body are available. Importantly, the technique and materials can be applied in standard multi-well plate configurations, allowing for high-throughput applications in cell culture, stem cell therapy, and drug discovery.

Patent status: pending.

Additional information:
A patent pending cell-stretching device is also available for licensing (please refer to case number HU 2983.) For Further Information Please Contact the Director of Business Development Vivian Berlin Email: [email protected] Telephone: (617) 495-0474

Inventor(s): Tschumperlin, Daniel J

Type of Offer: Licensing



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