In Vitro Differentiation of CD34+ Progenitor Cells into T-Lymphocytes (formerly

Summary Harvard Medical School researchers have developed a method for the production of large quantities of T lymphocytes in vitro. A monolayer of non-human primate thymic stromal cells is cocultured in vitro with human hematopoietic T cell progenitor cells, resulting in the differentiation and growth of mature human T cells. The T cells may be isolated from the stromal monolayer at any stage of differentiation and further expanded, allowing the production of large quantities of cells at a selected stage.


Advantages of this in-vitro differentiation method:


-Replicates the complex process of T lymphocyte differentiation more closely than any existing technique.


-Provides the relatively large number of T cells necessary for laboratory analysis and therapeutic use.


-Provides the capability to support in vitro T cell differentiation using a reagent that is available in relatively large amounts, minimizing inter-assay variation and enhancing quality control.

Applications -Internal Research and Development: Cells produced by this method can be used in the laboratory evaluation of drugs and cytokines that affect growth or differentiation of T cells.


-Kits: The non-human primate thymic stromal cells together with instructions for coculturing a sample of T cell progenitor cells could be provided as a kit.


-Gene Therapy: The method can be used for the ex vivo production of genetically altered T lymphocytes for introduction into a patient. For Further Information Please Contact the Director of Business Development Michal Preminger Email: [email protected] Telephone: (617) 432-0920

Inventor(s): Johnson, R. Paul

Type of Offer: Licensing



Next Patent »
« More Pharmaceutical Patents

Share on      


CrowdSell Your Patent