Multiplexing long DNA syntheses
Summary The invention enables multiplex gene synthesis in a single reaction, using very low concentrations of oligonucleotides. The method, which employs a unique temporary tagging strategy, offers significant advantages in terms of speed and economy over existing methods for building genes, and even genomes, particularly when coupled with high-throughput, high-density (e.g., array-based) oligonucleotide synthesis. The method also is useful for rapid generation of libraries of variant sequences for functional screening and/or selection. A U.S. patent application is pending, and international rights are available. The invention is available for either field-exclusive or non-exclusive license.
Applications DNA synthesis (e.g., whole genes or sets of genes). For Further Information Please Contact the Director of Business Development Maryanne Fenerjian Email: email@example.com Telephone: (617) 432-0920
Church, George M.
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