A Tool for the Linkage and Presentation of Multiple Protein Functional Domains

Summary Recent molecular biology research is moving beyond genetics to look at protein - protein interactions and how cells interact or communicate. These exciting areas are in need of improved tools that allow these interactions to be identified and studied.

Harvard Medical School researchers have developed a novel approach that allows investigators to quickly generate platforms for the multivalent display of protein domains. By the use of a cassette expression system, researchers can quickly insert active protein domains (enzymes, receptors, receptor ligands, antigens, etc.) into either the amino or carboxy terminus of a protein that forms a rigid octameric scaffold. An extension of the system provides the capability of covalently linking the hybrid peptides to inert support matrices, other peptides, oligonucleotides, or other macromolecules such as nucleic acids.

ADVANTAGES:

Easy construction of protein hybrids.

Multimeric.

Applications Ligand Presentation Framework. a system that enables the presentation of multiple copies of the same or a mixture of ligands in a conformationally constrained structure.

Multimeric solid phase linker. a system to immobilize multiple ligands to solid phases for signal amplification, purification etc.

Cell surface receptor studies. study cell surface receptors, cellular interactions, cell surface signaling etc.

Process chemistry, link two or more enzymes in linked partway. For Further Information Please Contact the Director of Business Development Katie Gordon Email: katherine_gordon@hms.harvard.edu Telephone: (617) 432-0920

Inventor(s): Hogle, James M.

Type of Offer: Licensing



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