Recombinant Baculovirus that Produces Human Topoisomerase I

Introduction Eukaryotic topoisomerase I (Topo I) is capable of relaxing both negatively and positively supercoiled DNA. The enzyme catalyzes changes in the superhelical state of duplex DNA by transiently breaking a single strand. Technology description Dr James Champoux’s laboratory has generated recombinant baculovirus that express wild type and active-site mutant (Y723F) versions of the full-length human Topo I and a 70-kDa protein (Topo70) which is missing the first 174 NH2-terminal amino acids, in addition to a recombinant baculovirus that expresses a NH2- and COOH-terminally truncated 58-kDa protein (Topo58). The specific activity of the full-length and the Topo70 enzymes are indistinguishable from the native human Topo I. Related Publication(s)
J Biol Chem 1996. 271(13):7593-7601 For more information on this technology contact:
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