Choreographed, Active Protein Delivery from Biodegradable Devices
Tracheal epithelial cells were earlier cultured in vitro to yield a model that near-perfectly replicates naturally epithelialized tracheal lumen vide technology no. 5413. Technologies no. 5546 and 5558 studied how bio-degradable polymers and degradat ion processes could be used in replanting tracheal epithelial tissue. To implant and successfully integrate epithelial cells within tracheal lumen, the nature and delivery kinetics of participant proteins from degradable biopolymers need to be under stood and controlled. This invention studies how protein structures/functions change within specific biopolymers, ways to control the holding/releasing of proteins, concentrations at which active proteins are released and overriding protocols to stab ilize/release proteins. The values are:
A 2-layer architecture of biopolymers allowed for choreographed release of 2 proteins [Keratinocyte growth factor (KGF) and Transforming Growth Factor alpha (TGF-?)].
Various bio-polymer embodimen e screened and synthesized to get best results; The addition of lead to the polymer blends released KGF and TGF in ways strikingly similar to natural processes; several viable lead based polymer blends were developed.
Protein release kinetics be varied by 2 orders of magnitude by varying molecular weights of the polymers, enabling control over the release kinetics.
The device can be used with any kind of protein (as also other biological entities), can release active proteins, and ynthesized using a single and cost effective process. The invention applies to the market of tracheal injuries (due to intubation, chemicals, chemotherapy, etc), to the study and research of re-epithelialization in other areas of the body (skin, c
Categories: Materials & Chemicals, Biomedical Engineering
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