Method for Ribozyme-catalyzed tRNA aminoacylation (2)
This invention is part of a set of novel processes to isolate, concentrate and reprogram enabling catalysts in protein synthesizing biochemical reactions. These help synthesize in vitro targeted proteins (natural/artificial) with application specific traits. Invention no. 5590 described a bifunctional ribozyme (and a superior process to prepare it) to catalyze in vitro synthesis of natural/unnatural aminoacyl-tRNA. This invention describes the synthesis of any desired aminoacyl-tRNA with the sai d catalyst. Done in two simple steps (aminoacylation of tRNA and facile purification), it is vastly more scalable than current methods and its protocol needs no special techniques. Current methods are labor-intensive and expensive, with complex multi ple steps of organic reactions, HPLC purification, and enzyme reactions. Other advantages are:
a)The described method greatly enhances the productivity (yield) and quality of the desired molecule over current ones. b)The synthesized molecules ca n be used directly for protein synthesis. c)Purified aminoacyl-tRNA can be used in both in vitro and in vivo translations. d)Productivity, quality and convenience of protein engineering are greatly enhanced.
This technology benefits research ins titutions and companies conducting experiments on cloning, recombinant technology, genetic research, protein research and more fundamental enquiries. Faster in vitro synthesis of a much larger number of proteins, protein derivatives and other peptide s will mean answers to several questions in huge secondary markets of drugs and pharmaceuticals, neutraceuticals, environment, forensic science, etc., by no means an exhaustive list.
Categories: Research Tool, Genomics Patents Issues: 7,001,723
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