tRNA aminoacylation on ribozyme-immobilized reins
This invention is part of a set of novel processes that isolate, concentrate and reprogram enabling catalysts in protein synthesizing biochemical reactions. These help synthesize in vitro targeted proteins (natural/artificial) with structural and fun ctional traits dictated by application necessity. Earlier inventions no. 5590 and 5610 described a bi-functional ribozyme that aminoacylated tRNA in a pool. Current methods for aminoacylation use a ribozyme that can be employed only once and also tak es time to dissociate itself from the tRNA. This invention immobilizes the ribozyme on an inexpensive resin, packs the resin into a column, adds the amino acid and tRNA and shakes it for about half an hour. When the resin is washed off, aminoacyl-tRN A remains with the immobilized ribozyme that can then be isolated.
a)The hydrazide resin used produces a strong covalent linkage making the catalyst durable. b)The ribozyme can be used several times over, saving costs and time. The immobilizatio n does not affect the catalytic ability in solution. c)Yields from this new method are comparable to the earlier solution method.
Potential implications of this technology envelop research institutions and companies conducting experiments on clon ing, recombinant technology, genetic research, protein research and more fundamental enquiries. Faster in vitro synthesis of a large number of proteins, protein derivatives and peptides imply solutions in huge secondary markets of drugs and pharmaceu ticals, neutraceuticals, environment, forensic science, etc., by no means an exhaustive list.
Categories: Research Tool, Genomics
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