A Simplified System for Generating Recombinant Adenoviruses

Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. A strategy, which simplifies the generation and production of such viruses is reported here. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulation, employing homologous recombination in bacteria rather than in eukaryotic cells. Following transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent protein. Homogeneous viruses can be obtained form this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes.

Inventor(s): Vogelstein, Bert

Type of Offer: Licensing



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