PECAM-1 Related Compositions and Associated Methods of Use
PECAM-l (CD 31), a member of the IG superfamily, is expressed strongly at endothelial cell junctions, leukocytes and platelets. PECAM-l has been suggested to play a role in angiogenesis, vasculogenesis and is critical in mediating the transendothelial migration of monocytes. This is an important first step in atherosclerosis as well as in inflammatory processes. However, similar reagents to abrogate cell adhesion and transendothelial migration are not available. Now, JHU scientists have designed and revised a polyclonal antibody against a peptide sequence resembling a portion of the 5th extracellular domain of PECAM-1 (named the Johns Hopkins Singapore-7 antibody (JHS-7 Ab). Experiments have shown that JHS-7 Ab can detect various human vascular cells including human umbilical vein endothelial cells; monocytes and platelets. JHS-7Ab can inhibit thrombin and induces release of Ca 2+. In addition, the antibody inhibits adhesion and transendothelial migration of monocytes. Description (Set) Proposed Use (Set) A PECAM-l domain specific antibody will prove useful in the detection of various isoforms of PECAM -1. Moreover, the antibody may be useful in abrogating cell adhesion in vitro and in vivo.
Chatterjee, Subroto B.
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