Automated Fluorometric Bioaerosol Concentration and Detection System
In the field of bioaerosol detection, there is a distinct need for an inexpensive, low power, robust trigger used to alert inhabitants of an increase in aerosol activity, thereby allowing the triggering of more sophisticated systems to determine the source. The JHU/APL concept, similar to the ringing of a doorbell, could be used to initiate operational countermeasures in both the battlefield and building protection applications.
The JHU/APL technology is an approach, which is significantly cheaper, smaller, requires less power and provides a simple output to trigger secondary, more sophisticated equipment for confirmation and agent identification. The technology builds on a recently developed and proven aflatoxin biosensor which utilizes a flash lamp and photo multiplier tube coupled with miniaturized fluidics to repeatedly sense small amounts of fluorescence in a two-minute cycle. This system couples a new high-flow, aerosol concentrator with the proven fluidics and fluorescence detection system in a single integrated unit for testing. The detection capabilities of the biosensor in its current form range from 0.1ppb to ppm, depending on sample volume. The integrated system should retain current detection capabilities, obtaining positive signals from as little as 10 Agent Containing Particles per Liter of Air (ACPLA) Other instruments have been extensively tested and implemented as a robust trigger in both inside and outside environments, however most instruments such as these are large, very expensive, and require a large power supply. In addition, these instruments typically give too much information, complicating the results and require complex algorithms to determine relatively little information.
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