Novel Technique for 3-Dimensional Multi-layer Growth of Anchorage Dependent Mammalian Cells

Mammalian cells have conventionally been grown as monolayers on solid surfaces of plastic or glass dishes and flasks or on microcarriers. They proliferate and spread to cover the surface, but stop growing when the surface is covered by only a monolayer of cells. This invention discloses a system that supports multilayer growth of anchorage dependent mammalian cells on a variety of extracellular matrices. Cells grown with this method grow at rates comparable to conventional methods, but can reach densities greater than nineteen layers of cells in tissue like structures and continue to secrete high amounts of cell-specific proteins. This invention offers the advantage of more continuous and normal cell-cell contact thus supporting better growth and a higher degree of differentiation than previously possible. Incorporated into the invention are specially designed user friendly molded plastic multi-well plates.

The worldwide cell culture market is estimated at $1.02 billion in 2005 and is expected to grow at a compound annual rate of 12.7% to reach more than $1.86 billion by 2010. With advances in product development, demand for specialty and custom serum-free media, particularly in biomanufacturing applications will drive market growth.

Stage of Development
*United States Patent No. 5,702,949. Novel System for Multilayer Growth of Anchorage-Dependent Cells, by Edward M. Trujillo and Catherine Rappaport, was issued Dec 30, 1997.
*A PCT patent application PCT/US01/47061. Method and Apparatus for Multi-Layer Growth of Anchorage-Dependent Cells has been filed with the World Intellectual Property Organization (International Publication Number WO 02/44341).
*This developing research is currently available under any sort of licensing or sponsored research arrangement.

Additional Info
*Rappaport, Catherine, Yvonne Rensch, Masoud Abbasi, Michael Kempe, Christian Rocaboy, John Gladysz and Edward M. Trujillo, New Perfluorocarbon System for Multilayer Growth of Anchorage Dependent Mammalian Cells. BioTechniques Vol. 32, no. 1, pp. 142-151, January 2002.
*Rappaport, Catherine, Edward M. Trujillo and Lih-Farn Soong, Novel Oxygenation System Supports Multilayer Growth of HeLa Cells BioTechniques, Vol. 21, no. 4, pp. 672-677, October, 1996.
*See Ed Trujillo�s research web site at

Inventor(s): Christian Rocaboy, Yvonne Rensch, Catherine Rappaport, Michael Kempe, Masoud Abbasi, John Gladysz, Edward Trujillo

Type of Offer: Licensing

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