Multiplexing Protein Binding Microarrays for Genetic Analysis
BACKGROUND: Our ability to understand and practically benefit from the control logic encoded in the human genome is currently limited by a lack of accurate information regarding promoter regulation. Gene regulatory proteins play critical roles in converting the genome into the complex ensemble of expressed genes which define variations in cell types and human health. Currently available antibody and surface Plasmon resonance-based methods have provided unprecedented insights into the promoter-specific binding of single regulatory proteins; however, these methods are unlikely to address the complexities inherent to eukaryotic transcriptional complexes, etc.
DESCRIPTION: Researchers at the University of California, Santa Barbara, have developed high-throughput and antibody–free multiplexed protein binding microarray (“mPBM”) to investigate protein-DNA interactions. These new tools use a combination of total internal reflectance fluorescent (TIRF), quantum dot enhanced TIRF and surface enhance Raman spectroscopy (SERS). The microarray allows an understanding of what factors control transcription in different states, the combinatorial challenge of complexes of proteins that bind and regulate transcription and provide applications towards understanding gene regulation. It can also screen small molecules for selective disruption of protein:protein interactions.
* Simultaneous end point scanning for multiple proteins across double-stranded DNA microarray
* Does not require antibodies or complicated wash regimens
* Optimized for specific and robust protein binding detection
* Allows real-time kinetic analysis
* Compatible with existing array printers and scanners
* Research Tool
* Biomarker Discovery
* Drug Discovery and Development
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