Fluorescence Lifetime and Fluorescence Assays
The invention provides a method for determining a degree of phosphorylation of a substrate, for example a peptide substrate, using a fluorescence probe that acts alone or with another material and has a lifetime that varies when in proximity to a phosphate, the method comprising: causing the fluorescence probe to fluoresce; measuring a time response of the fluorescence, and analysing the fluorescence time response to identify a fluorescence component having a lifetime associated with phosphorylated substrate and a fluorescence component having a lifetime associated with un-phosphorylated substrate.
GRAY ALEXANDER [GB]; PATERSON MICHAEL JOHN [GB]; GAKAMSKY DMITRY [GB]
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