Areas Dominika Elmlund is Knowledgeable in:
molecular biology and biochemistry of transcription and cancer
structural biology (crystallography/electron microscopy)
Techniques Dominika Elmlund Uses:
- thinking before doing
- define the main question and the expected outcome (vision)
- gather knowledge
- sort knowledge/information into useful and not useful
- get to know collaborators and their expertise to delegate parts of the project accordingly
- talk to people outside of the field to get a unbiased view, it's not good to be to close to the problem, diversity in the team is important
- brainstorm alone and in the group
- define a set of orthogonal approaches and go to the bench to test
- continue to work on promising ones, but do not stick to only one
- try and fail, it's a lot of information in failed experiments
- design negative and positive controls
- be creative
Dominika Elmlund's Problem Solving Skills:
- single-particle cryo-electron microscopy of biological macromolecules - specialist in computational method development, cryo sp
- protein crystallography - crystal seutp, screening, synchrotron, data analysis
- oligos purification from polyacrylamide gels
- genetic manipulation of yeast and bacteris
- protein overexpression
- molecular cloning
- DNA purification from yeast and bacteria cells
- affinity tag protein purification
- protein crosslinking
- chromatography methods
- native protein purification
- mass spectrometry
- protein modifications (phosphorylation etc.)
- PCR
- DNA modifications
- enzymatic assays development
- sequencing gel
- working with radioactivity, P32-UTP and P32-ATP
- SDS-PAGE
- dot and western blot
- audioradiography
- DNA agarose gels
- native gels - EMSA
- scientific writing (grants, publications, reviews)
- supervision of students, PhD students and post docs
Dominika Elmlund's Problem Solving Experience:
- - I design and supervise a wide range of of scientific project, from molecular biology/biochemistry to method development for cryo-electron microscopy
- published a number of scientific papers with high impact on the field