Instruments: AKTA Pilot/Explorer FPLC (GE Health care), TFF system (GE, Pall), 200L-scale TFF skid (Pall), Microfluidizer, HPLC (Agilent 1200, Hewlet Packard), Hollow-fiber filtration, Fermentors (New Brunswick Scientific model and Xcellerex disposable model), Chemiluminometer/Fluorometer(The Wallac 1420 VICTOR3), Ultracentrifuge, Image processor(Molecular Dynamics), Kodak Image Station 440, DNA sequencer (manual, automatic, capillary), UV and fluorescence spectrometer, Gas Chromatography.
Protein Chemistry: Protein purification with HPLC and FPLC using conventional columns (DEAE, Heparin, Hydroxyapatite, Gel filtration, C-18 hydrophobic columns etc) from brain, liver, and different cell types. Immunoprecipitation, ECL, Antibody prep and Western blot, ELISA, SDS-electrophoresis (PAGE), Silver Staining, Prep Electrophoresis, Dialysis, Peptide purification by FPLC.
Molecular Biology: Preparation of recombinant DNA, DNA sequencing (manual and automatic), PCR, qPCR, cDNA library construction and screening by Phage Display, In vitro transcription, Preparation of point mutations (PCR, Quick change method) and deletion mutants, DNA purification, PCR cloning, Plasmid construction, Phage display and Monoclonal Antibody engineering, Generation of stable cell lines, mRNA purification and northern blot, Construction of artificial genes for expression (150 - 1,400 nucleotides), Yeast two-hybrid, DNA hybridization, Genotyping, Gateway cloning.
Recombinant protein expression characterization and purification: E. coli, Bacillus anthracis, mammalian cells, and insect cells systems.
DNA Delivery Systems: Mammalian cells using Sindbis and Vaccinia virus. Knowledgeable of many methods of transfection, such as electroporation, vesicle, and calcium phosphate methods.
10 and 30 liter fermenters.
200 and 500 liter disposable fermentors
Lipid Biochemistry: Extraction and Purification of neutral and phospholipids with TLC and HPLC SAX column and their analysis.
Languages Chunghee Lee speaks: