Problem Solver
Dan Piestun 
Proven Solver
Areas Dan Piestun is Knowledgeable in:
Immune detection of proteins and proteomics. Cancer stem cells, Agrobacterium tumefaciens plant transformation, Plant Virology, plant and animal cell molecular biology, Bioinformatics, development of molecular markers for animal breeding.
Techniques Dan Piestun Uses:
business idea validation/ go-to-market strategy
Dan Piestun's Problem Solving Skills:
- immunology based diagnostics
- recalcitrant plant Agrobacterium mediated transformation (woody and herbaceous)
- bioinformatics
- molecular marker assisted animal breedinig
- protoplast culture and viral transfection
- retrovirus animal cell transformation
- plant genetic engineering
- entrepreneurship/ business model
- molecular diagnostics
- Virology
Dan Piestun's Problem Solving Experience:
- using tumor cell lines and fibroblast I demonstrated the oncogenic potential of the gene Nanog with the aid of MMLV (retrovirus) transformation vector, and evaluation of transcripts through Afymetrix microarray platform and bioinformatic tools.
- I have developed a succesful transformation system for Citrus plants through Agrobacterium tumefaciens
- I delivered bioinformatic solutions in a "bioinfo-hub", uniting four independent researach groups working on Rose petal genomics, ,proteomics, metabolomics and genetic engineering of flower plants.
- I developed molecular markers aimed for fertility traits in mammals (Bos taurus) for MAS.
- I practiced protoplast culture and viral transfection with CTV, regularly on Nicotiana benthamiana protoplast culture of control and transgenic lines to study viral dynamics in viral tolerant candidate plants.