Problem Solver
Imen Rabhi
Areas Imen Rabhi is Knowledgeable in:
-recombinant protein design and selection
-heterologous expression systems
-host-pathogen interection studies
-heterologous expression systems
-host-pathogen interection studies
Techniques Imen Rabhi Uses:
- Polymerase chain reaction, Site directed mutagenesis techniques for the study of protein-protein interaction and protein structure-function.
- Protein purification techniques.
- enhancing recombinant protein solubilization: protein design, clones design, and expression system used.
-Cellular culture techniques: Interferon Bioassays for biological activity determination.
-Using Affymetrix microarray technology to study the effect of parasite infection on the macrophage genes expression.
-Using of Ingenuity Pathways Analysis(R)software to decipher the cellular and metabolic pathways altered following parasite infection.
-clustering of expressed gene by the mean of dChip clustering software.
- Protein purification techniques.
- enhancing recombinant protein solubilization: protein design, clones design, and expression system used.
-Cellular culture techniques: Interferon Bioassays for biological activity determination.
-Using Affymetrix microarray technology to study the effect of parasite infection on the macrophage genes expression.
-Using of Ingenuity Pathways Analysis(R)software to decipher the cellular and metabolic pathways altered following parasite infection.
-clustering of expressed gene by the mean of dChip clustering software.
Imen Rabhi's Problem Solving Skills:
- host-pathogen interaction: in the context of transcriptomic studies
- mouse and human transcriptomic studies: microarray
- Molecular biology methods
- protein purification
- PCR- based Site-Directed Mutagenesis Method
- recombinant protein expression systems: E.coli and Pichia.pastoris
- host-pathogen interaction: in the context of host metabolic pathways studies
Imen Rabhi's Problem Solving Experience:
- - study of the effect of leishmania parasite on the host-macrophage gene expression.
-Using of Ingenuity Pathways Analysis(R)software to decipher the cellular and metabolic pathways altered following parasite infection.
-clustering of expressed gene by the mean of dChip clustering software - - I developed a new site-directed mutagenesis method based on the polymerase chain reaction
- My Phd work yielded two international patents procedure for the production of recombinant human interferon alpha in E.coli and in the yeast Pichia.pastoris heterologous expression systems.
- I used gene optimization and protein engineering design strategies for the production of recombinant human or rat proteins
-Training allowing the Transfer of technology from the National Institute for Biological Standards and Control, UK “Immunology and Endocrinology Division”
Subject: Interferon Bioassays for biological activity determination.