Problem Solver
Janusz Zukowski
Techniques Janusz Zukowski Uses:
- Practical knowledge of analytical sciences particularly separation sciences
- Separation techniques used HPLC, CE, GC, multi dimensional HPLC, micro HPLC, multimodal HPLC, Counter Current Chromatography
- Chromatographic method development for different forms of drug substances and its related compounds
- Developed methodology for chiral separations
- HPLC detection modes used: UV-Vis, fluorescence, MS and others
- Impurity isolation by classical extraction, solid phase extraction and preparative chromatography
- Chromatographic method evaluation and validation
- Separation techniques used HPLC, CE, GC, multi dimensional HPLC, micro HPLC, multimodal HPLC, Counter Current Chromatography
- Chromatographic method development for different forms of drug substances and its related compounds
- Developed methodology for chiral separations
- HPLC detection modes used: UV-Vis, fluorescence, MS and others
- Impurity isolation by classical extraction, solid phase extraction and preparative chromatography
- Chromatographic method evaluation and validation
Janusz Zukowski's Problem Solving Skills:
- analytical chemistry problem solving
Janusz Zukowski's Problem Solving Experience:
- - Determination of unnatural amino acids in human body fluids. This involved a fluorescence derivatisation, column switching separation and fluorescence detection. For the first time a large amounts of D-pipecolic acid in the urine
and plasma of subjects with peroxisomal deficiencies were detected. - - A polar organic mobile phase methodology now widely used in chiral HPLC separations all over the world was developed.
- - For the first time enantiomers separation of hydrocarbons (alpha-, beta-pinenes and camphene, compounds having no functional group) using HPLC were achieved.
- - A number of HPLC methods were developed for GSK which are widely used all over the organisation.
- - Chiral paroxetine HPLC method developed and is in use in Global Manufacturing Supply. It was published in “Chirality” journal and in US Pharmacopeia.
- - Development of fingerprinting HPLC methodology for detection at low parts per trillion of route-specific impurities in Clavulanate/Amoxicillin formulations. This was used to defend GlaxoSmithKline's Intellectual Property. A
number of competitors Clavulanate/Amoxicillin products were analysed.