Problem Solver
Lindsey Silva
Areas Lindsey Silva is Knowledgeable in:
Virology: Herpesviruses
Cellular biology: nuclear structure/function, autophagy
Chromatin remodeling: epigenetic regulation of gene expression
Cellular biology: nuclear structure/function, autophagy
Chromatin remodeling: epigenetic regulation of gene expression
Techniques Lindsey Silva Uses:
-Cloning strategies for optimization of viral protein expression in tissue culture system
-Confocal microscopy for visualization of cellular processes
-Chromatin Immunoprecipitation and real-time PCR to determine chromatin regulation on cellular and viral gene promoters
-Confocal microscopy for visualization of cellular processes
-Chromatin Immunoprecipitation and real-time PCR to determine chromatin regulation on cellular and viral gene promoters
Lindsey Silva's Problem Solving Skills:
- Powerpoint and poster presentation skills of data at national conferences
- Handle mouse work
- Critical reading, writing, and analysis of data for publication in peer-reviewed journals
- Handle biosafety level two pathogens in tissue culture
- Basic statistics: T-test
- Composed, edited, submitted, and received NIH funding for research: Peter A. Jone's training grant
- Radioactivity training with 32-P and 35-S isotopes
Lindsey Silva's Problem Solving Experience:
- I implemented a tissue culture system for chromatin immunoprecipitation and immunofluorescence studies to study Herpes Simplex Virus genome localization in the host cell nucleus.
- I optimized expression of Kaposi's sarcoma viral GPCR protein in tissue culture to screen for small molecule antagonists to identify potential drug targets